Real-time nucleic acid sequence-based amplification (NASBA) assay targeting MIC1 for detection of Cryptosporidium parvum and Cryptosporidium hominis oocysts

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• 作者：Birgitte K. Hø ; nsvall^a ; ^b ; ^{birgitte.honsvall@hbv.no} ; Lucy J. Robertson^c ; ^{lucy.robertson@nmbu.no}
• 关键词：MIC1 ; RT-NASBA ; Cryptosporidium ; Detection ; Viability assessment ; Species differentiation
• 刊名：Experimental Parasitology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：172
• 期：Complete
• 页码：61-67
• 全文大小：348 K
• 卷排序：172

文摘

Primer sets targeting MIC1 in C. parvum and C. hominis made for real-time NASBA. C. parvum and C. hominis oocysts detected down to 5 oocysts in 10 μl. C. parvum oocysts distinguished from C. hominis oocysts using this NASBA assay. MIC1 transcripts detected in both living and inactivated oocysts.