Efficient regeneration and genetic transformation platform applicable to five Musa varieties

详细信息    查看全文

• 作者：Juhua Liu^a ; ¹ ; ^{juhua69@126.com} ; Pengzhao Gao^a ; ¹ ; Xiuxiu Sun^c ; ¹ ; Jing Zhang^a ; ¹ ; Peiguang Sun^b ; Jiashui Wang^b ; Caihong Jia^a ; Jianbin Zhang^a ; Wei Hu^a ; Biyu Xu^a ; ^{biyuxu@126.com} ; Zhiqiang Jin^a ; ^b ; ^{jinzhiqiang@itbb.org.cn}
• 关键词：Agrobacterium-mediated transformation ; Banana (Musa spp.) ; Direct organogenesis ; Functional genomics of banana ; Gus ; Particle bombardment ; Staple food ; Transformation system for banana
• 刊名：Electronic Journal of Biotechnology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：25
• 期：Complete
• 页码：33-38
• 全文大小：971 K
• 卷排序：25

文摘

Banana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Conventional breeding has been seriously hampered by their long generation time, polyploidy, and sterility of most cultivated varieties. Establishment of an efficient regeneration and transformation system for banana is critical to its genetic improvement and functional genomics.ResultsIn this study, a vigorous and repeatable transformation system for banana using direct organogenesis was developed. The greatest number of shoots per explant for all five Musa varieties was obtained using Murashige and Skoog medium supplemented with 8.9 μM benzylaminopurine and 9.1 μM thidiazuron. One immature male flower could regenerate 380–456, 310–372, 200–240, 130–156, and 100–130 well-developed shoots in only 240–270 d for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Longitudinal sections of buds were transformed through particle bombardment combined with Agrobacterium-mediated transformation using a promoterless β-glucuronidase (GUS) reporter gene; the highest transformation efficiency was 9.81% in regenerated Gongjiao plantlets in an optimized selection medium. Transgenic plants were confirmed by a histochemical assay of GUS, polymerase chain reaction, and Southern blot.ConclusionsOur robust transformation platform successfully generated hundreds of transgenic plants. Such a platform will facilitate molecular breeding and functional genomics of banana.