Adipose tissue-derived stem cells ameliorates dermal fibrosis in a mouse model of scleroderma

详细信息    查看全文

• 作者：Wei Chen¹ ; ² ; ³ ; ^# ; Zhi-Kuan Xia³ ; ^# ; Man-Hui Zhang³ ; Gui-Chun Ding³ ; Xiao-Yan Zhang³ ; Zheng-Xu Wang³ ; Rong-Ya Yang³ ; ^{rongyay@yeah.net}
• 关键词：Adipose-derived stem cells ; Limited cutaneous scleroderma ; Mouse model ; VEGF ; TGF-β1
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：10
• 期：1
• 页码：52-56
• 全文大小：308 K
• 卷排序：10

文摘

To investigate the therapeutic potential of adipose-derived stem cells (ADSCs) for limited cutaneous scleroderma (LS) in mouse models.MethodsADSCs were isolated from pathogen-free female C57BL/6 mice and LS was induced in wild type (WT) C57BL/6 mice via daily injection of bleomycin (0.1 mL × 300 μg/mL) for 4 weeks; then the ADSCs were subcutaneously injected into the dorsal area in the model treatment group, and 100 μL of phosphate-buffered saline (PBS) solution was injected into the same site in the model control group. Green fluorescent protein (GFP) was used to track the cells using an in vivo imaging system on days 7, 14, 21, and 28 after transplantation. All mice were sacrificed and histologic analyses were performed after 4 weeks, and the skin thickness, collagen deposition and the total content of hydroxyproline were evaluated. Additionally, immunohistochemistry were performed to compare the tissue expression and distribution of TGF-β1 and VEGF between the ADSCs treatment group and the treatment control group.ResultsWT C57BL/6 LS mouse model were successfully established and GFP in vivo fluorescence imaging showed that the translated ADSCs survived at the local for at least 4 weeks. Compared with the control group, the ADSCs treatment group significantly attenuated bleomycin-induced dermal fibrosis, reduced the skin thickness and the total content of hydroxyproline (P < 0.05). The ADSCs treatment group displayed significantly lower levels of TGF-β1 and higher levels of VEGF than the control group (P < 0.05).ConclusionsADSCs may provide a feasible and practical treatment for autoimmune diseases such as LS and ameliorate dermal fibrosis.