Characterization of Irreversible Electroporation Ablation with a Validated Perfused Organ Model

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• 作者：Suyashree Bhonsle ; MS^a ; ^b ; Mohammad Bonakdar ; PhD^a ; ^c ; Robert E. Neal II ; PhD^d ; ^e ; Charles Aardema ; BS^a ; John L. Robertson ; VMD ; PhD^a ; Jonathon Howarth ; BS^a ; Helen Kavnoudias ; BMedSci ; PhD^d ; Kenneth R. Thomson ; MBChB ; MD^d ; S. Nahum Goldberg ; MD ; MSc^f ; Rafael V. Davalos ; PhD^a ; ^c ; ^{davalos@vt.edu}
• 关键词：IRE ; irreversible electroporation ; TTC ; triphenyl tetrazolium chloride
• 刊名：Journal of Vascular and Interventional Radiology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：27
• 期：12
• 页码：1913-1922.e2
• 全文大小：2459 K
• 卷排序：27

文摘

To develop and validate a perfused organ model for characterizing ablations for irreversible electroporation (IRE)–based therapies.Materials and MethodsEight excised porcine livers were mechanically perfused with a modified phosphate-buffered saline solution to maintain viability during IRE ablation. IRE pulses were delivered using 2 monopolar electrodes over a range of parameters, including voltage (1,875–3,000 V), pulse length (70–100 µsec), number of pulses (50–600), electrode exposure (1.0–2.0 cm), and electrode spacing (1.5–2.0 cm). Organs were dissected, and treatment zones were stained with triphenyl tetrazolium chloride to demonstrate viability and highlight the area of ablation. Results were compared with 17 in vivo ablations performed in canine livers and 35 previously published ablations performed in porcine livers.ResultsAblation dimensions in the perfused model correlated well with corresponding in vivo ablations (R2 = 0.9098) with a 95% confidence interval of < 2.2 mm. Additionally, the validated perfused model showed that the IRE ablation zone grew logarithmically with increasing pulse numbers, showing small difference in ablation size over 200–600 pulses (3.2 mm ± 3.8 width and 5.2 mm ± 3.9 height).ConclusionsThe perfused organ model provides an alternative to animal trials for investigation of IRE treatments. It may have an important role in the future development of new devices, algorithms, and techniques for this therapy.