Laboratory diagnosis of specific antibody deficiency to pneumococcal capsular polysaccharide antigens by multiplexed bead assay

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• 作者：Heleen Borgers ; Leen Moens ; Capucine Picard ; Axel Jeurissen ; Marc Raes ; Kate Sauer ; Marijke Proesmans ; Kris De Boeck ; Jean-Laurent Casanova ; Isabelle Meyts ; Xavier Bossuyt
• 关键词：Immune deficiency ; Streptococcus pneumoniae ; Specific anti-polysaccharide immune deficiency
• 刊名：Clinical Immunology
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：134
• 期：2
• 页码：198-205
• 全文大小：489 K

文摘

We evaluated a multiplexed bead-based assay (xMAP® Pneumococcal Immunity assay from Luminex) for the simultaneous determination of antibodies against 14 capsular polysaccharides. Post-vaccination (Pneumovax®) antibody concentrations were measured in 35 healthy children, 40 healthy adults, 99 consecutive patients with increased susceptibility to respiratory infection, and 24 patients with a deficient anti-polysaccharide antibody response. The serotype-specific lower 5th percentile (cutoff) value for the post-immunization antibody concentration was determined in healthy individuals. Eleven of 99 patients (11 % ) failed to mount a response that was > 5th percentile of controls for at least 6 of the 14 serotypes tested, whereas only 3 of 75 controls (4 % ) failed to do so. All patients with known anti-polysaccharide antibody deficiency failed to mount a response that was > 5th percentile of controls for at least 6 of the 14 serotypes tested. The XMAP® pneumococcal immunity panel appears useful for identifying individuals with a low response to the unconjugated pneumococcal vaccine.