Characterization of proteases and nucleases associated with ethylene-induced programmed cell death in immature cucumber fruit
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- 作者：Jin Su Leea ; Brandon M. Hurrb ; brandon.hurr@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Donald J. Hubera ; C.Eduardo Vallejosa ; Steven A. Sargenta
- 关键词：Cucumber ; Ethylene ; Protease ; Nuclease ; Programmed cell death ; Watersoaking
- 刊名：Postharvest Biology and Technology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：110
- 期：Complete
- 页码：190-196
- 全文大小：1083 K
文摘
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PCD-related proteases and nucleases are described in ethylene-treated cucumber fruit.
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2 Bifunctional nucleases, 4 RNases and 3 proteases were highly ethylene-responsive.
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All ethylene-responsive nucleases were Ca2+-dependent and reducing-agent sensitive.
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Bifunctional nucleases had alkaline pH optima, RNases acidic, and proteases acidic.
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Protease and nuclease increases preceded loss of cell integrity and watersoaking.