Estimating the variation in S phase duration from flow cytometric histograms

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• 作者：Sara Larsson ; Tobias Ryden ; Ulla Holst ; Stina Oredsson ; Maria Johansson
• 关键词：Branching process ; Cell cycle progression ; S phase duration ; Flow cytometry
• 刊名：Mathematical Biosciences
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：213
• 期：1
• 页码：40-49
• 全文大小：425 K

文摘

A stochastic model for interpreting BrdUrd DNA FCM-derived data is proposed. The model is based on branching processes and describes the progression of the DNA distribution of BrdUrd-labelled cells through the cell cycle. With the main focus on estimating the S phase duration and its variation, the DNA replication rate is modelled by a piecewise linear function, while assuming a gamma distribution for the S phase duration. Estimation of model parameters was carried out using maximum likelihood for data from two different cell lines. The results provided quite a good fit to the data, suggesting that stochastic models may be a valuable tool for analysing this kind of data.