Cell membrane morphology analysis using an infrared sensor system

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• 作者：Sander van den Driesche^a ; ^{sdriesche@uni-bremen.de}Author Vitae ; Filippo Iuliano^bAuthor Vitae ; Christoph Haiden^cAuthor Vitae ; Daniela Pucciarelli^dAuthor Vitae ; Heimo Breiteneder^dAuthor Vitae ; Silvia Pastorekova^bAuthor Vitae ; Christine Hafner^dAuthor Vitae ; Michael J. Vellekoop^aAuthor Vitae
• 关键词：Cell membrane ; Infrared sensor ; Label-free ; Membrane morphology
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：31 March, 2013
• 年：2013
• 卷：179
• 期：Complete
• 页码：150-156
• 全文大小：872 K

文摘

In this paper, we report on the CH₂-symmetric to CH₂-antisymmetric stretch ratio as a method to determine membrane morphology alterations in mammalian cells. Cell membrane morphology is an important parameter for tumour development studies and for determining the lowest effective dose of antitumour agents, reducing the occurrence of side effects. For this study a normal epithelial kidney cell line and three human melanoma cell lines were investigated. The epithelial kidney cell line Madin-Darby Canine Kidney was exposed to the cholesterol reduction agent methyl-¦Â-cyclodextrin. The cholesterol reduction changes the cell membrane morphology and the plasma membrane of the cells become less rigid. A thin layer chromatography analysis was performed into test the efficiency of the cholesterol reduction method.

The antitumour agent cisplatin is known to cause the opposite effect and makes the cell membrane more rigid. In order to investigate the cell membrane morphology change three human melanoma cell lines were treated with cisplatin.

For the CH₂-stretch ratio measurements we used a self-designed infrared sensor system, which has the potential to be further developed in a point-of-care instrument. With the sensor system the morphology changes due to cholesterol reduction and cisplatin treatments were established.

This study proves that the CH₂-symmetric to CH₂-antisymmetric stretch ratio can be successfully applied for label-free cell membrane morphology analysis.