Zerumbone induces apoptosis in T-acute lymphoblastic leukemia cells

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• 作者：Siddig Ibrahim Abdelwahab^a ; ^{siddigroa@yahoo.com} ; Ahmad Bustamam Abdul ; ^a ; ^{abustamam@putra.upm.edu.my} ; Syam Mohan^a ; Manal Mohamed Elhassan Taha^a ; Suvitha Syam^a ; Mohamed Yousif Ibrahim^a ; Abdelbasit Adam Mariod^a
• 关键词：Apoptosis ; Scanning electron microscope ; Zerumbone ; TUNEL assay
• 刊名：Leukemia Research
• 出版年：2011
• 出版时间：February 2011
• 年：2011
• 卷：35
• 期：2
• 页码：268-271
• 全文大小：440 K

文摘

Zerumbone (ZER) is a potential anticancer natural compound, isolated from Zingiber zerumbet Smith. In this investigation, the anticancer properties of ZER were evaluated on cancer cells of T-acute lymphoblastic leukemia, CEM-ss. The results showed that ZER has cytotoxic effect against CEM-ss cells with an IC₅₀ of 8.4 ± 1.9 μg/ml (coefficient of variation < 30 % ). Comparatively, 5-fluorouracil (positive control), imposed an inhibitory effect on CEM-ss cells with an IC₅₀ of 1.94 ± 0.06 μg/ml. Scanning electron microscopy (SEM) results revealed abnormalities such as membrane blebbing, holes and cytoplasmic extrusions, all of which are characteristics of apoptosis. In addition, ZER has increased the number of TUNEL-positive stain and the cellular level of caspase-3, the hallmarks of apoptosis, on treated CEM-ss cells. It could be concluded that, ZER was able to produce apoptosis on T-acute lymphoblastic leukemia, CEM-ss. The current findings suggest that ZER might be helpful for improving the usefulness of anticancer agents in the therapy of leukemia.