Sensitive determination of plasma protein binding of cationic drugs using mixed-mode solid-phase microextraction
详细信息 查看全文
- 作者：Hester Peltenburga ; H.Peltenburg@uu.nl" class="auth_mail" title="E-mail the corresponding author ; Ingrid J. Bosmanb ; Joop L.M. Hermensa
- 关键词：Free concentration ; Plasma protein binding ; Organic cations ; Mixed-mode SPME ; Ionized pharmaceuticals ; Cation exchange
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2015
- 出版时间：10 November 2015
- 年：2015
- 卷：115
- 期：Complete
- 页码：534-542
- 全文大小：1095 K
文摘
- •
C18/SCX SPME was used to measure free concentrations of ionized pharmaceuticals.
- •
Cfree can be used to calculate binding affinities to bovine and human serum albumin.
- •
Fouling effects were observed and quantified but had limited effects on binding affinities.
- •
Kinetic and equilibrium SPME resulted in similar calculated binding affinities.
- •
C18/SCX SPME application allows sensitive determination of protein binding of cations.