Method development and validation for the quantitation of the complement inhibitor Cp40 in human and cynomolgus monkey plasma by UPLC-ESI-MS

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• 作者：Alexandra Primikyri^a ; Malvina Papanastasiou^a ; Yiannis Sarigiannis^a ; Sophia Koutsogiannaki^a ; Edimara S. Reis^a ; Joel V. Tuplano^b ; Ranillo R.G. Resuello^b ; Bo Nilsson^c ; Daniel Ricklin^a ; ¹ ; ^{ricklin@upenn.edu} ; ^{d.ricklin@unibas.ch} ; John D. Lambris^a ; ^{lambris@upenn.edu}
• 关键词：C3G ; C3 glomerulopathy ; CF ; correction factor ; IS ; internal standard ; LLOQ ; lower limit of quantitation ; NHP ; non-human primate ; PD ; pharmacodynamics ; PK ; pharmacokinetic ; PNH ; paroxysmal nocturnal hemoglobinuria ; QC ; quality control ; SPE ; solid-phase extraction
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：1041-1042
• 期：Complete
• 页码：19-26
• 全文大小：1233 K
• 卷排序：1041

文摘

A sensitive quantitation method for the complement inhibitor Cp40 in human and primate plasma is developed and validated. The quantitation is based on solid-phase extraction and UPLC–ESI–MS analysis. The developed method was found to be linear, accurate and precise. The validated method was successfully applied to a pharmacokinetic profiling study of Cp40 in cynomolgus monkeys.