Canine influenza viruses with modified NS1 proteins for the development of live-attenuated vaccines

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• 作者：Aitor Nogales^a ; Kai Huang^b ; Caroline Chauché ; ^c ; Marta L. DeDiego^a ; ^d ; Pablo R. Murcia^c ; Colin R. Parrish^b ; Luis Martí ; nez-Sobrido^a ; ^{luis_martinez@urmc.rochester.edu}
• 关键词：Influenza A virus ; Canine influenza virus ; Inactivated influenza vaccines ; Live-attenuated influenza vaccines ; Non-structural protein 1 ; Canine tracheal explant ; Reverse genetics ; NS1 deficient influenza viruses
• 刊名：Virology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：500
• 期：Complete
• 页码：1-10
• 全文大小：1467 K
• 卷排序：500

文摘

We have generated by reverse genetics H3N8 CIVs containing truncated or a deleted NS1 protein. NS1 mutant H3N8 CIVs replicated efficiently in MDCK cells, important for vaccine production. H3N8 CIV with mutated NS1 are attenuated in vivo (mice) or ex vivo (canine tracheal explants). NS1 mutant viruses confer complete protection against challenge with WT H3N8 CIV. This is the first description of a LAIV for the prevention and control of H3N8 CIV in dogs.