Engineering the central biosynthetic and secondary metabolic pathways of Pseudomonas aeruginosa strain PA1201 to improve phenazine-1-carboxylic acid production
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- 作者：Kaiming Jin1 ; Lian Zhou1 ; Haixia Jiang ; Shuang Sun ; Yunling Fang ; Jianhua Liu ; Xuehong Zhang ; Ya-Wen He ; yawenhe@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Phenazine-1-carboxylic acid ; Phenazine biosynthetic pathway ; Chorismate-utilizing enzymes ; Secondary metabolism ; Pseudomonas aeruginosa PA1201
- 刊名：Metabolic Engineering
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：32
- 期：Complete
- 页码：30-38
- 全文大小：2764 K
文摘
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The secondary metabolite phenazine-1-carboxylic acid (PCA) is active against a wide range of fungal plant pathogens, and was developed as a new biopesticide.
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10">The PCA-producing strain PA1201 was modified via a combined strategy involving gene deletion and replacement, promoter engineering and protein engineering.
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15">A total of 478 genes responsible for virulence factor production, secondary metabolite production, PCA biosynthesis and efflux pumping were engineered.
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The genetically stable strain PA-IV exhibited a 54.6-fold PCA yield increase over PA1201, producing 9882 mg/L of PCA in fed-batch fermentation.