Abiotic stress and phytohormones affect enzymic activity of 1-O-(indole-3-acetyl)-β-d-glucose: myo-inositol indoleacetyl transferase from rice (Oryza sativa)

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• 作者：Anna Ciarkowska ^{annaciar@doktorant.umk.pl" class="auth_mail" title="E-mail the corresponding author} ; Maciej Ostrowski ; ^{maciejost@umk.pl" class="auth_mail" title="E-mail the corresponding author} ; Anna Jakubowska ^{anjakubo@umk.pl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：2 ; 4-D ; 2 ; 4-dichlorophenoxyacetic acid ; ABA ; abscisic acid ; GA3 ; gibberellic acid ; GH3 ; Gretchen Hagen3 ; IAA ; indole-3-acetic acid ; IAGlc ; 1-O-indole-3-acetyl-β-d-glucose ; IAInos ; indole-3-acetyl-myo-inositol ; JA ; jasmonic acid ; MeJA ; methyl jasmonate ; NAA1 ; naphtalene acid ; SA ; salicylic acid ; SCPL ; serine carboxypeptidase-like ; UDP ; guridine diphosphate glucose
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：20 October 2016
• 年：2016
• 卷：205
• 期：Complete
• 页码：93-96
• 全文大小：755 K

文摘

Indole-3-acetic acid (IAA) conjugation is a part of mechanism regulating free auxin concentration. 1-O-(indole-3-acetyl)-β-d-glucose: myo-inositol indoleacetyl transferase (IAInos synthase) is an enzyme involved in IAA-ester conjugates biosynthesis. Biotic and abiotic stress conditions can modulate auxin conjugates formation in plants. In this study, we investigated effect of plant hormones (IAA, ABA, SA and 2,4-D) and abiotic stress (drought and salt stress: 150 mM NaCl and 300 mM NaCl) on expression level and catalytic activity of rice IAInos synthase. Enzymic activity assay indicated that all tested phytohormones affected activity of IAInos synthase, but only ABA had inhibiting effect, while IAA, SA and 2,4-D activated the enzyme. Drought and salt stress induced with lower NaCl concentration resulted in decreased activity of IAInos synthase, but 300 mM NaCl had no effect on the enzyme. Despite observed differences in enzymic activities, no changes of expression level, tested by semiquantitative RT-PCR and Western blot, were detected. Based on our results it has been supposed that plant hormones and stress conditions affect IAInos synthase activity on posttranslational level.