The sequence polymorphisms of the HVI and HVII regions were determined by PCR and direct sequencing. The haplotypes found were checked by phylogenetic haplogroup analysis to decrease haplotype assignation errors and to avoid artificial recombination.
We found 78 different haplogroups in this set of samples. A high percentage of haplotypes (53 % ) belong to European haplogroups due to the large flow of European immigrants from colonial times. However, we also observed a high percentage of haplotypes that belong to Amerindian haplogroups (39 % ), which were conserved through the female Amerindian population contribution. Furthermore, we found a small group of haplotypes with Sub-Saharan African origin (3.5 % ) due to the slave trade at the beginning of Argentina's colonization.
The sequences found showed that this set of samples has an abundant haplogroup diversity because of the European and Amerindian ethnic group contribution.