Reduction of misleading (鈥渇alse鈥? positive results in mammalian cell genotoxicity assays. III: Sensitivity of human cell types to known genotoxic agents
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- 作者：Paul Fowlera ; pauljamesfowler@gmail.com" class="auth_mail ; Robert Smithb ; Katie Smithb ; Jamie Younge ; Laura Jeffreyb ; Paul Carmichaela ; David Kirklandc ; Stefan Pfuhlerd
- 关键词：Genotoxicity ; Human cells ; Misleading positive results ; Sensitivity
- 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- 出版年：June 2014
- 年：2014
- 卷：767
- 期：Complete
- 页码：28-36
- 全文大小：423 K
文摘
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Study compared micronucleus response in human cell types TK6, HuLy and HepG2 cells with 17 well characterised genotoxic chemicals.
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TK6 and HuLy have been shown to be highly sensitive with 88% detected as positive in the in vitro micronucleus test (15 out of 17).
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Using TK6 or HuLy cells will reduce “false” or “misleading” positive incidence in the in vitro micronucleus test without compromising sensitivity.