A novel bio-nanocomposite based on hemoglobin and carboxyl graphene for enhancing the ability of carrying oxygen

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• 作者：Yang LiuAuthor Vitae ; Jin GongAuthor Vitae ; Weiping WuAuthor Vitae ; Yuting FangAuthor Vitae ; Qi WangAuthor Vitae ; Haiying Gu ; ^{hygu@ntu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{guhy99@21cn.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Hemoglobin ; Carboxyl graphene ; Electrochemistry ; Oxygen-carrying ability
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：222
• 期：Complete
• 页码：588-597
• 全文大小：2280 K

文摘

Hemoglobin (Hb) was successfully combined with carboxyl graphene (CG) through condensation reaction to form novel bio-nanocomposite hemoglobin-carboxyl graphene bio-nanocomposites (CG-Hb). Scanning electron microscope (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Raman spectra indicated that the morphology and structure of CG-Hb. Cyclic voltammograms of CG-Hb modified on the gold nanoparticles (Au-NPs) electro-deposited on glassy carbon electrode (GCE) exhibited a pair of redox peaks (−0.235 V and −0.122 V vs. SCE) and showed excellent biosensor for hydrogen peroxide, which realized the direct electron transfer between CG-Hb and GCE. The electrochemistry properties and the ability of oxygen-carrying were investigated by electrochemical methods. It was found this kind of bio-nanocomposite maintained the natural biological activity of Hb and was explored to be no observable denaturation, which can be considered as a novel effective oxygen carrier in electrochemical aspect for further research.