Usefulness of a rapid immuno-migration test for the detection of canine monocytic ehrlichiosis in Africa

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• 作者：Bernard Davoust ; Daniel Parzy ; Jean-Paul Demoncheaux ; Rapha毛l Tine ; Mamadou Diarra ; Jean-Lou Mari茅 ; Oleg Mediannikov
• 关键词：Dog ; Ehrlichia canis ; Indirect immunofluorescence test ; Rapid immunomigration ; Immunochromatography ; Diagnostic
• 刊名：Comparative Immunology, Microbiology and Infectious Diseases
• 出版年：January, 2014
• 年：2014
• 卷：37
• 期：1
• 页码：31-37
• 全文大小：573 K

文摘

A rapid immuno-migration test for the serological detection of canine monocytic ehrlichiosis, Witness^庐 Ehrlichia (WE) (Zoetis, France), was evaluated in 528 serum samples from dogs living in endemic areas of West and East Africa: Senegal (N = 208), Ivory Coast (N = 7), Sudan (N = 27), and Djibouti (N = 286). Of these dogs, 200 were French military working dogs (MWD) temporarily residing in Africa. The WE test results were compared with those obtained by indirect immunofluorescence (IFA). The sensitivity of WE was 97% [94.2, 98.7] with a specificity of 100% [98.6, 100]. In MWD, the seroprevalence (IFA) was 7%; in native dogs, it reached 77.1%. This significant difference can be explained by prophylactic measures from which MWD benefit. The WE test represents a simple, fast and reliable test for the detection of anti-Ehrlichia canis antibodies. Its implementation for the diagnosis of clinical cases has been validated in the field, and its use allows easy detection of asymptomatic dogs that may be carriers of E. canis.