Exploiting DNA mini-barcodes as molecular markers to authenticate saffron (Crocus sativus L.)
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- 作者：Caterina Villa ; Joana Costa ; Liliana Meira ; M. Beatriz P.P. Oliveira ; Isabel Mafra ; isabel.mafra@ff.up.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：Saffron ; Authentication ; DNA barcode ; ITS1 ; ITS2 ; matK ; HRM analysis ; Sequencing
- 刊名：Food Control
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：65
- 期：Complete
- 页码：21-31
- 全文大小：3288 K
文摘
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ITS1, ITS2 and matK as candidates barcode loci for Crocus spp. differentiation.
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Real-time PCR coupled to high resolution melting (HRM) analysis.
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HMR analysis targeting ITS1 and matK loci were Crocus genus specific.
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ITS2 locus was specific of Crocus sativus L. and Crocus cartwrightianus.
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DNA-barcoding with HRM analysis as a reliable tool for saffron authentication.