Study on chemical analysis, antioxidant and in vitro antifungal activities of essential oil from wild Vitex agnus-castus L. seeds growing in area of Argan Tree of Morocco against clinical strains of Candida responsible for nosocomial infections

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• 作者：A. Asdadi^a ; ^{asdadiali@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; A. Hamdouch^a ; A. Oukacha^b ; R. Moutaj^c ; S. Gharby^d ; H. Harhar^d ; M. El Hadek^e ; B. Chebli^f ; L.M. Idrissi Hassani^a
• 关键词：Vitex agnus-castus L. ; Essential oil of seeds ; Nosocomial infections ; Candida species ; Antioxidant activities ; Antifungal ; GC/MS
• 刊名：Journal de Mycologie Médicale / Journal of Medical Mycology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：25
• 期：4
• 页码：e118-e127
• 全文大小：507 K

文摘

To study the composition, the antioxidant activity and the in vitro antifungal action anti-Candida species of essential oils extracted from seeds of Vite xagnus-castus L.

Materials and method

The essential oils were extracted using Clevenger-type apparatus and analyzed by gas chromatography/mass spectrometry (GC/MS). The antioxidant activity was analyzed using the DPPH free radical-scavenging method. Susceptibility tests for Candida albicans (12), C. dubliniensis (1), C. glabrata (3), C. krusei (3), C. parapsilosis (6), C. lusitaniae (1), C. famata (1) and C. tropicalis (3) were expressed as inhibition zone by the disc-diffusion method and as minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) by the broth macrodilution method, compared to amphotricin B and fluconazol as standard drugs.

Results

Major components were: 1,8-cineole (19.61%), sabinene (14.57%), α-pinene (9.76%), β-farnesene (6.04%), β-caryophyllene oxide (5.83%) and β-caryophyllene (5.02%). A low antioxidant activity was found (IC₅₀ = 1.072 mg/ml), but it can be exploited. V. agnus-castus seeds essential oils disosed a 35-58 mm zone of inhibition (mean: 49 mm) against all 30 isolates tested. In broth macrodilution method, all the tested Candida species were susceptible to the essential oils and this activity was concentration-dependent. MIC values varied from 0.13 to 2.13 mg/ml V. agnus-castus seeds essential oils.

Conclusion

Results of this study indicated that the oils of plant origin could be used as potential anti-Candida species causative agents of nosocomial infections. These oils exhibited a noticeable antifungal activity against the selected fungi. The natural antifungal substances are inexpensive and have fewer side effects, they may represent alternative therapies for candidiasis.