Old age and the associated impairment of bones' adaptation to loading are associated with transcriptomic changes in cellular metabolism, cell-matrix interactions and the cell cycle

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• 作者：Gabriel L. Galea ; PhD^a ; ¹ ; Lee B. Meakin ; PhD^a ; ¹ ; ^{lee.meakin@bristol.ac.uk} ; Marie A. Harris ; PhD^b ; Peter J. Delisser ; BVSc(Hons)^a ; Lance E. Lanyon ; DSc^a ; Stephen E. Harris ; PhD^b ; Joanna S. Price ; PhD^a
• 关键词：MAPK ; mitogen-activated protein kinase ; Actn2 ; actinin alpha 2 ; AMPK ; adenosine monophosphate-activated protein kinase ; Atf3 ; activating transcription factor 3 ; ATP ; Adenosine triphosphate ; b2MG ; Beta-2-microglobulin ; Bcl11a ; B-cell CLL/lymphoma 11A ; Bcl212 ; Bcl-2-like protein 2 ; BiNGO ; A Biological Network Gene Ontology tool ; BMD ; bone mineral density ; BMP ; bone morphogenetic protein ; Ccna2 ; cyclin A2 ; Ccnd3 ; cyclin D3 ; Cdc20 ; cell division cycle 20 ; Cdca3 ; cell division cycle associated 3 ; Cdk
• 刊名：Gene
• 出版年：2017
• 出版时间：30 January 2017
• 年：2017
• 卷：599
• 期：Complete
• 页码：36-52
• 全文大小：3481 K
• 卷排序：599

文摘

In old animals, bone's ability to adapt its mass and architecture to functional load-bearing requirements is diminished, resulting in bone loss characteristic of osteoporosis. Here we investigate transcriptomic changes associated with this impaired adaptive response. Young adult (19-week-old) and aged (19-month-old) female mice were subjected to unilateral axial tibial loading and their cortical shells harvested for microarray analysis between 1 h and 24 h following loading (36 mice per age group, 6 mice per loading group at 6 time points). In non-loaded aged bones, down-regulated genes are enriched for MAPK, Wnt and cell cycle components, including E2F1. E2F1 is the transcription factor most closely associated with genes down-regulated by ageing and is down-regulated at the protein level in osteocytes. Genes up-regulated in aged bone are enriched for carbohydrate metabolism, TNFα and TGFβ superfamily components. Loading stimulates rapid and sustained transcriptional responses in both age groups. However, genes related to proliferation are predominantly up-regulated in the young and down-regulated in the aged following loading, whereas those implicated in bioenergetics are down-regulated in the young and up-regulated in the aged. Networks of inter-related transcription factors regulated by E2F1 are loading-responsive in both age groups. Loading regulates genes involved in similar signalling cascades in both age groups, but these responses are more sustained in the young than aged. From this we conclude that cells in aged bone retain the capability to sense and transduce loading-related stimuli, but their ability to translate acute responses into functionally relevant outcomes is diminished.