Neurobasal media facilitates increased specificity of siRNA-mediated knockdown in primary cerebellar cultures
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- 作者：Julie Ry Gustafssona ; julie-jacobsen@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; julie.jacobsen@regionh.dk" class="auth_mail" title="E-mail the corresponding author ; Georgia Katsioudia ; 1 ; gkatsioudi@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Shohreh Issazadeh-Navikasb ; 1 ; shohreh.issazadeh@bric.ku.dk" class="auth_mail" title="E-mail the corresponding author ; Birgitte Rahbek Kornuma ; c ; 1 ; birgitte.kornum@regionh.dk" class="auth_mail" title="E-mail the corresponding author
- 关键词：CGNs ; cerebellar granule neurons ; Ara-C ; cytosine β-d-arabinofuranoside hydrochloride ; NBM ; Neurobasal&trade ; media
- 刊名：Journal of Neuroscience Methods
- 出版年：2016
- 出版时间：1 December 2016
- 年：2016
- 卷：274
- 期：Complete
- 页码：116-124
- 全文大小：1709 K
文摘
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Accell siRNAs was tested in cerebellar granule neurons in two media.
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siRNAs delivered in Accell Delivery media caused off-target effects.
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Specific downregulation of target was obtained in Neurobasal™ media.
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The off-target effect was not a secondary response to downregulation of target.
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