Protection of donor lung inflation in the setting of cold ischemia against ischemia-reperfusion injury with carbon monoxide, hydrogen, or both in rats

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• 作者：Chao Meng^a ; ^f ; Liangjuan Ma^b ; Li Niu^c ; Xiaoguang Cui^a ; ^f ; Jinfeng Liu^a ; ^f ; Jiyu Kang^d ; Rongfang Liu^a ; ^f ; Jingchun Xing^a ; ^f ; Changlin Jiang^e ; Huacheng Zhou^d ; ^{zhouhuacheng@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Carbon monoxide ; Hydrogen ; Lung inflation ; Cold ischemia phase ; Ischemia-reperfusion injury ; Lung transplantation
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 April 2016
• 年：2016
• 卷：151
• 期：Complete
• 页码：199-206
• 全文大小：2089 K

文摘

Lung ischemia-reperfusion injury (IRI) may be attenuated through carbon monoxide (CO)'s anti-inflammatory effect or hydrogen (H₂)'s anti-oxidant effect. In this study, the effects of lung inflation with CO, H₂, or both during the cold ischemia phase on graft function were observed.

Materials and methods

Rat donor lungs, inflated with 40% oxygen (control group), 500 ppm CO (CO group), 3% H₂ (H₂ group) or 500 ppm CO + 3% H₂ (COH group), were kept at 4 °C for 180 min. After transplantation, the recipients' artery blood gas and pressure-volume (P-V) curves were analyzed. The inflammatory response, oxidative stress and apoptosis in the recipients were assessed at 180 min after reperfusion.

Key findings

Oxygenation in the CO and H₂ groups were improved compared with the control group. The CO and H₂ groups also exhibited significantly improved P-V curves, reduced lung injury, and decreased inflammatory response, malonaldehyde content, and cell apoptosis in the grafts. Furthermore, the COH group experienced enhanced improvements in oxygenation, P-V curves, inflammatory response, lipid peroxidation, and graft apoptosis compared to the CO and H₂ groups.

Significance

Lung inflation with CO or H₂ protected against IRI via anti-inflammatory, anti-oxidant and anti-apoptotic mechanisms in a model of lung transplantation in rats, which was enhanced by combined treatment with CO and H₂.