Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate

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• 作者：Mingjie Jin ; William Bothfeld ; Samantha Austin ; Trey K. Sato ; Alex La Reau ; Haibo Li ; Marcus Foston ; Christa Gunawan ; Richard D. LeDuc ; John F. Quensen ; Mick Mcgee ; Nirmal Uppugundla ; Alan Higbee ; Ruwan Ranatunga ; Charles W. Donald ; Gwen Bone ; Arthur J. Ragauskas ; James M. Tiedje ; Daniel R. Noguera ; Bruce E. Dale ; et al.
• 关键词：Hydrolysate storage ; Hydrolysate characterization ; AFEX ; Hydrolysate fermentation ; Hydrolysate precipitate
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：147
• 期：Complete
• 页码：212-220
• 全文大小：1413 K

文摘

To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX? (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4 ¡ãC and pH 4.8, accounting for only 0.02 % of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH₄PO₄¡¤6H₂O) and brushite (CaHPO₄¡¤2H₂O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.