Label-free chemiluminescent strategy for highly selective and sensitive detection of adenosine triphosphate by cofactor-dependent enzymatic ligation-triggered polymerase chain reaction
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- 作者：Lu-Yan Yaoa ; bAuthor Vitae ; Xiao-Qian Yua ; bAuthor Vitae ; Rui-Jin YucAuthor Vitae ; Yan-Jun Zhaoa ; bAuthor Vitae ; Ai-Ping Fana ; b ; aipingfan1980@163.com" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：ATP-dependent enzymatic reaction ; Rolling circle amplification ; Adenosine triphosphate ; Graphene oxide ; Chemiluminescence ; Label free
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：238
- 期：Complete
- 页码：175-181
- 全文大小：1099 K
文摘
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A label-free CL assay system was developed for the detection of ATP.
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The method is high sensitive with a detection limit of 0.03 nM.
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The system showed high specificity to ATP against its analogues.
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ATP was determined in diluted serum indicating the applicability of this assay.
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