Quantitative analysis of gene expression changes in response to genotoxic compounds

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• 作者：Ceri A. Morris^a ; ^b ; ^{morrisc10@cardiff.ac.uk} ; Gamal A. El-Hiti^c ; Ian Weeks^b ; Stuart Woodhead^d ; Keith Smith^e ; Peter Kille^a
• 关键词：Acridinium-ester ; Chemiluminescence ; Genotoxic ; Cystatin A (CSTA) ; RAD51C ; Tumour suppressor p53 (TP53)
• 刊名：Toxicology in Vitro
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：15-28
• 全文大小：2124 K
• 卷排序：39

文摘

Development of in vitro assays to measure 3 genes (RAD51C, CSTA, TP53). Expression profiles in response to exposure of HEPG2 cells to 18 compounds CSTA dysregulation was the most effective indicator of exposure. Optimisation of a novel method for chemiluminescent detection of nucleic acid targets New format is capable of detecting gene expression levels in cell lines.