DNA biosensor based on the electrochemiluminescence of Ru(bpy)₃²⁺ with DNA-binding intercalators

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• 作者：Jeong-Gun Lee ; Kyusik Yun ; Guei-Sam Lim ; Sang Eun Lee ; Suhyeon Kim ; Je-Kyun Park
• 关键词：Electrochemiluminescence (ECL) ; Tris-(2 ; 2′ ; -bipyridyl)ruthenium(II) ; DNA-binding intercalator ; 4′ ; 6-diamidino-2-phenylindole (DAPI) ; DNA biosensor ; DNA hybridization detection
• 刊名：Bioelectrochemistry
• 出版年：2007
• 出版时间：May 2007
• 年：2007
• 卷：70
• 期：2
• 页码：228-234
• 全文大小：926 K

文摘

This paper reports a novel detection method for DNA hybridization based on the electrochemiluminescence (ECL) of Ru(bpy)₃²⁺ with a DNA-binding intercalator as a reductant of Ru(bpy)₃³⁺. Some ECL-inducible intercalators have been screened in this study using electrochemical methods combined with a chemiluminescent technique. The double-stranded DNA intercalated by doxorubicin, daunorubicin, or 4′,6-diamidino-2-phenylindole (DAPI) shows a good ECL with Ru(bpy)₃²⁺ at + 1.19 V (versus Ag/AgCl), while the non-intercalated single-stranded DNA does not. In order to stabilize the self-assembled DNA molecules during ECL reaction, we constructed the ECL DNA biosensor separating the ECL working electrode with an immobilized DNA probe. A gold electrode array on a plastic plate was assembled with a thru-hole array where oligonucleotide probes were immobilized in the side wall of thru-hole array. The fabricated ECL DNA biosensor was used to detect several pathogens using ECL technique. A good specificity of single point mutations for hepatitis disease was obtained by using the DAPI-intercalated Ru(bpy)₃²⁺ ECL.