Detection EGFR exon 19 status of lung cancer patients by DNA electrochemical biosensor
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- 作者：Xiong-Wei Xua ; Xiu-Hua Wenga ; shirly0409@163.com" class="auth_mail" title="E-mail the corresponding author ; Chang-Lian Wanga ; Wei-Wei Lina ; Ai-Lin Liub ; Wei Chenb ; chenandhu@163.com" class="auth_mail" title="E-mail the corresponding author ; Xin-Hua Linb ; xhl1963@sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Epidermal growth factor receptor ; In-frame deletions ; Electrochemical biosensor ; Non-small cell lung carcinoma ; Detection ; Lambda exonuclease
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 June 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：411-417
- 全文大小：1198 K
文摘
Electrochemical biosensor for detection EGFR exon 19 status.
Different region around deletion hotspots was selected to design DNA probes.
Satisfactory discrimination when deletions in capture probe interaction region.
Highly-efficient ssDNA generation by lambda exonuclease digestion.
Discriminated wild and deletion type EGFR for lung cancer patients.