Autophagy-related genes Raptor, Rictor, and Beclin1 expression and relationship with multidrug resistance in colorectal carcinoma

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• 作者：Wu Shuhua ; MD^a ; ¹ ; ^{wsh6108@126.com" class="auth_mail" title="E-mail the corresponding author} ; Sun Chenbo ; MSc^b ; ¹ ; Li Yangyang ; MSc^a ; Gao Xiangqian ; MSc^a ; He Shuang ; MSc^a ; Li Tangyue ; MSc^b ; Tian Dong ; MD^a ; ^{dongtianbz@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Colorectal cancer ; MDR ; Raptor ; Rictor ; Beclin1 ; Prognosis
• 刊名：Human Pathology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：46
• 期：11
• 页码：1752-1759
• 全文大小：1121 K

文摘

This study aims to evaluate the relationship between the expressions of autophagy-related genes Raptor, Rictor, and Beclin1 and the expression of multidrug resistance (MDR) gene in colorectal cancer (CRC) patients. Immunohistochemistry and real-time polymerase chain reaction were used to detect the protein and messenger RNA expressions of mammalian target of rapamycin (mTOR), Raptor, Rictor, Beclin1, light chain 3 (LC3), and MDR-1 in 279 CRC specimens. Patients were followed up annually by telephone or at an outpatient clinic. Results revealed that the protein and messenger RNA expressions of Beclin1, LC3, mTOR, Raptor, Rictor, and MDR-1 in CRC are significantly higher than in adjacent tissues. LC3 expression in poorly differentiated CRC is higher than that in well-differentiated CRC, and the expression of mTOR, Raptor, Rictor, and LC3 in lymph node metastasis is higher than that obtained in the absence of lymph node metastasis. The expression of LC3 is positively correlated with those of Beclin1 and Rictor and negatively correlated with Raptor and mTOR in CRC. The expression of Raptor is negatively correlated with Rictor. The expression of MDR-1 is positively correlated with those of Beclin1, LC3, and Rictor and negatively correlated with Raptor and mTOR. Kaplan-Meier analysis revealed that the 5-year survival rate of patients without lymph node metastasis; positive expression of Rictor, Beclin1, and LC3; and negative expression of Raptor and mTOR were higher than those with these characteristics. To conclude, the expressions of Beclin1, Raptor, and Rictor are related to the development and progression of colorectal carcinoma and MDR. (Clinical trial registration number: 2014-009-01.)