Application of step-wise gradient high-performance counter-current chromatography for rapid preparative separation and purification of diterpene components from Pseudolarix kaempferi Gordon

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• 作者：Shichao He^a ; ^b ; Shucai Li^a ; Jianhong Yang^a ; Haoyu Ye^a ; Shijie Zhong^a ; Hang Song^b ; Yongkui Zhang^b ; Cheng Peng^c ; Aihua Peng^a ; ^c ; ^{peng_aihua@163.com} ; Lijuan Chen^a ; ^b ; ^{lijuan17@hotmail.com}
• 关键词：High-performance counter-current chromatography ; Pseudolarix kaempferi Gordon ; Gradient elution ; Diterpene
• 刊名：Journal of Chromatography A
• 出版年：2012
• 出版时间：27 April, 2012
• 年：2012
• 卷：1235
• 期：Complete
• 页码：34-38
• 全文大小：591 K

文摘

In general, simultaneously separation and purification of components with a broad polarity range from traditional Chinese medicine (TCM) is a challenge by an ordinary high-speed counter-current chromatography (HSCCC) method. In this paper, we describes a rapid and efficient separation method of combining three-step gradient elution and two-step flow-rate gradient elution using high-performance counter-current chromatography (HPCCC) to separate 8 diterpene compounds simultaneously within 80 min in a single run from the alcohol extract of Pseudolarix kaempferi Gordon. This separation process produced 166 mg pseudolaric acid B O-¦Â-d-glucopyranoside (PABGly), 152 mg pseudolaric acid C (PAC), 8 mg deacetylpseudolaric acid A (deacetylPAA), 5 mg pseudolaric acid A O-¦Â-d-glucopyranoside (PAAGly), 484 mg pseudolaric acid B (PAB), 33 mg pseudolaric acid B methyl ester (PAB methyl ester), 10 mg pseudolaric acid A (PAA) and 18 mg pseudolaric acid H (PAH) from 1.0 g crude sample with purities of 98.6 % , 99.6 % , 92.3 % , 92.2 % , 99.2 % , 99.4 % , 98.3 % , 91.0 % , respectively. Our study indicates that the suitable combination of step-wise gradient elution and flow-rate gradient elution using HPCCC is an effective strategy to separate complex components from natural products.