Species identification through pyrosequencing 12S rRNA gene

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• 作者：Zhen Hu^a ; ^b ; Zheng Wang^a ; Suhua Zhang^a ; Yingnan Bian^a ; Chengtao Li^a ; ^{lichengtaohla@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Species identification ; Pyrosequencing ; 12S rRNA gene
• 刊名：Forensic Science International: Genetics Supplement Series
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：5
• 期：Complete
• 页码：e561-e563
• 全文大小：398 K

文摘

The identification of the biological origin of a specimen is necessary in some forensic casework. Mitochondrial DNA (mtDNA) has become a useful tool in forensic identification because of the high number of copies and the lack of recombination. In this study, we amplified and sequenced a 100-bp fragment of the mtDNA 12S ribosomal RNA (12S rRNA) gene from 17 different species including human, monkey, chicken, duck, cattle, sheep, dog, SD rat, mouse, goose, pig, horse, cat, rabbit, donkey, pigeon, and deer. The amplified products were sequenced for the specimen identification by pyrosequencing technique. The results showed that the specimen origin could be accurately determined by only 12 bases. Through pyrosequencing the 12S rRNA gene, a new method for discrimination of 17 species was developed.