miR-613 inhibits proliferation and invasion of breast cancer cell via VEGFA

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• 作者：Junzhao Wu^a ; Peng Yuan^a ; Qixin Mao^a ; Peng Lu^b ; Tian Xie^a ; Hanzhao Yang^a ; Chengzheng Wang^a ; ^{wangchengzheng@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：miR-613 ; VEGFA ; Breast cancer
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2016
• 出版时间：9 September 2016
• 年：2016
• 卷：478
• 期：1
• 页码：274-278
• 全文大小：931 K

文摘

MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in breast cancer, has remained elusive. Here, we identified that miR-613 inhibits breast cancer cell proliferation by negatively regulates its target gene VEGFA. In breast cancer cell lines, CCK-8 proliferation assay indicated that the cell proliferation was inhibited by miR-613, while miR-613 inhibitor significantly promoted the cell proliferation. Transwell assay showed that miR-613 mimics significantly inhibited the migration and invasion of breast cancer cells, whereas miR-613 inhibitors significantly increased cell migration and invasion. Luciferase assays confirmed that miR-613 directly bound to the 3′ untranslated region of VEGFA, and western blotting showed that miR-613 suppressed the expression of VEGFA at the protein levels. This study indicated that miR-613 negatively regulates VEGFA and inhibits proliferation and invasion of breast cancer cell lines. Thus, miR-613 may represent a potential therapeutic molecule for breast cancer intervention.