Characterization of divergent pseudo-sucrose isomerase from Azotobacter vinelandii: Deciphering the absence of sucrose isomerase activity

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• 作者：Jong-Hyun Jung^a ; ^b ; Min-Ji Kim^a ; Woo-Soo Jeong^a ; Dong-Ho Seo^c ; Suk-Jin Ha^d ; Young Wan Kim^e ; Cheon-Seok Park^a ; ^{cspark@khu.ac.kr}
• 关键词：Sucrose isomerase ; Oligo 1 ; 6 glucosidase ; RLDRD motif ; Azotobacter vinelandii
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：29 January 2017
• 年：2017
• 卷：483
• 期：1
• 页码：115-121
• 全文大小：948 K
• 卷排序：483

文摘

Sucrose isomerase and oligo-1,6-glucosidase (O16G) belongs to the GH13_31 subfamily. Azotobacterglucocosidase (AZOG) is intermediary SIase harboring both RLDRD motif and O16G-like conserved region. AZOG exhibited O16G similar substrate specificity and intermolecular transglycosylation ability to synthesize isomaltose using methyl-α-glucoside. Shuffling with SIase resulted in change of substrate specificity of AZOG and revival of sucrose isomerase activity.