Differences in oral habit and lymphocyte subpopulation affect malignant transformation of patients with oral precancer

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• 作者：Chien-Yang Yeh^a ; ^h ; Chien-Ling Lin^b ; ^h ; Mei-Chi Chang^c ; ^d ; ^h ; ^{mcchang@mail.cgust.edu.tw" class="auth_mail" title="E-mail the corresponding author} ; Hsin-Ming Chen^a ; Seng-Heng Kok^a ; Shu-Hui Chang^e ; Ying-Shiung Kuo^f ; Liang-Jiunn Hahn^a ; Chiu-Po Chan^g ; Jang-Jaer Lee^a ; ^{leejj@ntuh.gov.tw" class="auth_mail" title="E-mail the corresponding author} ; Jiiang-Huei Jeng^a ; ^{jhjeng@ntu.edu.tw" class="auth_mail" title="E-mail the corresponding author}
• 关键词：lymphocyte population ; malignant transformation ; oral cancer ; oral habits
• 刊名：Journal of the Formosan Medical Association
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：115
• 期：4
• 页码：263-268
• 全文大小：212 K

文摘

In Taiwan, the combination of betel quid chewing, alcohol consumption, and smoking habits increases oral cancer risk by 123-fold compared to persons without these habits. Lymphocyte populations in patients may potentially affect the malignant transformation of oral precancer.

Methods

A total of 28 patients with oral precancer from our previous cohort were enrolled in this study, and their personal information and oral habits were documented. Their lymphocyte populations (CD4+, CD8+, CD19+, and CD56+) and activation markers (CD25 and CD69) were determined by flow cytometry from 1999 to 2004. After follow up till December 2014, data of patients with/without malignant transformation were recorded, and the relation between oral habits and percentage of initial lymphocyte markers was evaluated using the Student t test and Fisher's exact test.

Results

Ten precancer patients developed oral squamous cell carcinoma with a mean period of malignant transformation of 6.8 ± 2.1 years. Patients with malignant transformation had a mean age of 48.4 ± 5.0 years (n = 10), relatively more than that of patients without malignant transformation (41.6 ± 6.3 years, n = 18) (p < 0.05). An increase was noted in the population of peripheral blood mononuclear cells expressing CD4+CD69+, CD19+CD69+, and CD56+CD69+ (p < 0.05) in precancer patients with malignant transformation. Alcohol consumption showed an association with the malignant transformation of patients with precancer (p = 0.030), whereas betel quid and smoking showed little effect.

Conclusion

These results suggest that age, alcohol consumption, and early activation of T cells, B cells, and natural killer cells are crucial in the malignant transformation of oral precancer. Analysis of patient's lymphocyte populations may help predict the malignant transformation of oral precancer.