Gut and liver T-cells of common clonal origin in primary sclerosing cholangitis-inflammatory bowel disease

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• 作者：Eva Kristine Klemsdal Henriksen¹ ; ² ; ³ ; ⁴ ; Kristin Kaasen Jø ; rgensen¹ ; ⁵ ; Fatemeh Kaveh³ ; ⁴ ; ⁶ ; Kristian Holm¹ ; ² ; ³ ; ⁴ ; David Hamm⁷ ; Johanna Olweus³ ; ⁸ ; ⁹ ; Espen Melum¹ ; ² ; ³ ; ¹⁰ ; Brian K. Chung¹ ; ⁴ ; ¹³ ; Tor J. Eide⁴ ; ¹¹ ; Knut E.A. Lundin¹⁰ ; ¹² ; Kirsten Muri Boberg¹ ; ³ ; ⁴ ; ¹⁰ ; Tom H. Karlsen¹ ; ² ; ³ ; ⁴ ; ¹⁰ ; Gideon M. Hirschfield¹³ ; Evaggelia Liaskou¹³ ; ^{e.liaskou@bham.ac.uk}
• 关键词：High-throughput sequencing ; Human gut ; Human liver ; IBD ; PSC ; T cell receptor ; Ulcerative colitis ; Sclerosing cholangitis ; Inflammatory bowel disease
• 刊名：Journal of Hepatology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：66
• 期：1
• 页码：116-122
• 全文大小：727 K
• 卷排序：66

文摘

Recruitment of gut-derived memory T-cells to the liver is believed to drive hepatic inflammation in primary sclerosing cholangitis (PSC). However, whether gut-infiltrating and liver-infiltrating T-cells share T cell receptors (TCRs) and antigenic specificities is unknown. We used paired gut and liver samples from PSC patients with concurrent inflammatory bowel disease (PSC-IBD), and normal tissue samples from colon cancer controls, to assess potential T cell clonotype overlap between the two compartments.MethodsHigh-throughput sequencing of TCRβ repertoires was applied on matched colon, liver and blood samples from patients with PSC-IBD (n = 10), and on paired tumor-adjacent normal gut and liver tissue samples from colon cancer patients (n = 10).ResultsAn average of 9.7% (range: 4.7–19.9%) memory T cell clonotypes overlapped in paired PSC-IBD affected gut and liver samples, after excluding clonotypes present at similar frequencies in blood. Shared clonotypes constituted on average 16.0% (range: 8.7–32.6%) and 15.0% (range: 5.9–26.3%) of the liver and gut memory T-cells, respectively. A significantly higher overlap was observed between paired PSC-IBD affected samples (8.7%, p = 0.0007) compared to paired normal gut and liver samples (3.6%), after downsampling to equal number of reads.ConclusionMemory T-cells of common clonal origin were detected in paired gut and liver samples of patients with PSC-IBD. Our data indicate that this is related to PSC-IBD pathogenesis, suggesting that memory T-cells driven by shared antigens are present in the gut and liver of PSC-IBD patients. Our findings support efforts to therapeutically target memory T cell recruitment in PSC-IBD.Lay summaryPrimary sclerosing cholangitis (PSC) is a devastating liver disease strongly associated with inflammatory bowel disease (IBD). The cause of PSC is unknown, but it has been suggested that the immune reactions in the gut and the liver are connected. Our data demonstrate for the first time that a proportion of the T-cells in the gut and the liver react to similar triggers, and that this proportion is particularly high in patients with PSC and IBD.