Multiple, simultaneous, independent gradients for a versatile multidimensional liquid chromatography. Part II: Application 1 - Large increases in isoform resolution of human transferrin by use of dual simultaneous independent gradients of pH & acetonitrile on a mixed bed (anion exchange plus reversed phase) stationary phase
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- 作者：Latchezar I. Tsonev ; Allen G. Hirsh ; agh@cryobiophysica.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Multi-dimensional liquid chromatography ; pH gradient chromatography ; Protein chromatography ; Mixed bed stationary phases ; Human transferrin isoforms ; Protein folding intermediates
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：14 October 2016
- 年：2016
- 卷：1468
- 期：Complete
- 页码：173-182
- 全文大小：2267 K
文摘
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Creation of simultaneous independent pH & acetonitrile gradients in quad HPLC.
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Simultaneous pH & acetonitrile gradients work on mixed bed RP-IEX columns.
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pH & acetonitrile gradients on mixed bed RP-IEX columns are a new proteomics tool.
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More than 60 Human Transferrin isoforms are demonstrated.