High-performance thin-layer chromatography (HPTLC) for the simultaneous quantification of the cyclic lipopeptides Surfactin, Iturin A and Fengycin in culture samples of Bacillus species

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• 作者：Mareen Geissler^a ; Claudia Oellig^b ; Karin Moss^a ; Wolfgang Schwack^b ; Marius Henkel^a ; ^{marius.henkel@uni-hohenheim.de} ; Rudolf Hausmann^a
• 关键词：HPLC ; high-performance liquid chromatography ; HPTLC ; high-performance thin-layer chromatography ; LOD ; limit of detection ; LOQ ; limit of quantification ; MSM ; mineral salt medium
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：1044-1045
• 期：Complete
• 页码：214-224
• 全文大小：1447 K
• 卷排序：1044

文摘

A HPTLC method for the quantification of Surfactin, Iturin A and Fengycin is presented. A sample pretreatment protocol was established that enables further quantification of samples. The introduced HPTLC method was validated as being highly accurate and precise. The method is applicable to various Bacillus strains.