Capillary electrophoresis with online stacking in combination with AgNPs@MCM-41 reinforced hollow fiber solid-liquid phase microextraction for quantitative analysis of Capecitabine and its main metabolite 5-Fluorouracil in plasma samples isolated from cancer patients

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• 作者：Mehrdad Forough^a ; Khalil Farhadi^a ; ^{khalil.farhadi@yahoo.com} ; ^{kh.farhadi@urmia.ac.ir} ; Rahim Molaei^a ; Hedayat Khalili^a ; Ramin Shakeri^b ; Asghar Zamani^c ; Amir Abbas Matin^d
• 关键词：AgNPs ; Ag nanoparticles ; CAP ; Capecitabine ; 5-FU ; 5-Fluorouracil ; TP ; Thymidine phosphorylase ; CE ; Capillary electrophoresis ; 5-FUH2 ; Dihydro-5-fluorouracil ; FBAL ; α-Fluoro-β-alanine ; FUPA ; 5-Fluoro-ureido-propionic acid ; FLC ; Fluoropyrimidine carbamate ; 5-DFCR ; 5-deoxy-fluorocytidine ; 5-DFUR ; 5-deoxy-fluorouridine ; UV ; Ultra-violet ; MS ; mass spectrometry ; MS/MS ; tandem mass spectrometry ; LC ; Liquid chromatography ; LLE ; liquid&ndash ; liquid extraction ; LOD ; Limit of Detection ; LOQ ; Limit of Quant
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：1040
• 期：Complete
• 页码：22-37
• 全文大小：2683 K
• 卷排序：1040

文摘

A novel strategy based on AgNPs@MCM41-HF-SLPME-FASI-CE was developed and validated for the first time. Simultaneous quantitation of Capecitabine and its major metabolite (5-Fluorouracil) in human plasma was accomplished. AgNPs@MCM41-HF-SLPME was used for isolation of parent drug and metabolite from plasma. Validation procedure was based on FDA guideline and the results demonstrated satisfactory analytical performance data. The validated assay was used to determine the selected compounds in real samples collected from cancer patients.