Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography

详细信息    查看全文

• 作者：David V. McCalley ^{David.Mccalley@uwe.ac.uk}
• 关键词：Hydrophilic interaction chromatography ; HILIC ; Retention ; Selectivity ; Additives
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：3 February 2017
• 年：2017
• 卷：1483
• 期：Complete
• 页码：71-79
• 全文大小：1525 K
• 卷排序：1483

文摘

Anion retention in hydrophilic interaction liquid chromatography with TFA studied. Strong retention of cationic solutes with hydride columns in TFA is moderated. Unusual retention effects probably not caused by metal cation adsorption. Methane sulfonic acid gives cationic retention i.e. different selectivity to TFA. Ionic strength of mobile phase an important influential factor in these effects.