Straightforward approach to produce recombinant scorpion toxins—Pore blockers of potassium channels

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• 作者：Oksana Nekrasova^a ; ^b ; Ksenia Kudryashova^a ; ^b ; Arkadiy Fradkov^b ; Sergey Yakimov^b ; Maria Savelieva^a ; Mikhail Kirpichnikov^a ; ^b ; Alexey Feofanov^a ; ^b ; ^{avfeofanov@yandex.ru}
• 关键词：AgTx2 ; agitoxin 2 ; ChTx ; charybdotoxin ; CSTEV ; TEV protease cleavage site ; HgTx ; hongotoxin ; IPTG ; isopropyl β-d-1-thiogalactopyranoside ; KTx ; peptide blockers of potassium channels ; KTx1 ; kaliotoxin 1 ; MBP ; maltose-binding protein ; MgTx ; margatoxin ; ScTx ; scyllatoxin ; TEV ; tobacco etch virus protease ; RF HPLC ; reverse-phase HPLC
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：10 January 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：127-135
• 全文大小：1512 K
• 卷排序：241

文摘

Simple approach to production of peptide blockers of K+ channels was developed. Yields are 12–22 mg of peptide from 1 l of E. coli culture. Advantages of approach are demonstrated for 6 peptides from 4 subfamilies of blockers.