Separation of open-cage fullerenes using nonaqueous capillary electrophoresis

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• 作者：Hsiu-Li Su ; Wan-Chun Kao ; Cheng-yu Lee ; Shih-Ching Chuang ; You-Zung Hsieh
• 关键词：Nonaqueous capillary electrophoresis ; Open-cage fullerene ; Trifluoroacetic acid
• 刊名：Journal of Chromatography A
• 出版年：2010
• 出版时间：25 June 2010
• 年：2010
• 卷：1217
• 期：26
• 页码：4471-4475
• 全文大小：210 K

文摘

In this study, nonaqueous capillary electrophoresis (NACE) was used to separate three open-cage fullerenes. Trifluoroacetic acid (TFA) was used as the nonaqueous background electrolyte to change the analytes’ mobilities. The selectivity and separation efficiency were critically affected by the nature of the buffer system, the choice of organic solvent, and the concentrations of TFA and sodium acetate (NaOAc) in the background electrolyte. The optimized separation occurred using 200 mM TFA/20 mM NaOAc in MeOH/acetonitrile (10:90, v/v), providing highly efficient baseline separation of the open-cage fullerenes within 5 min. The migration time repeatability for the three analytes was less than 1 % (relative standard deviation). Thus, NACE is a rapid, useful alternative to high-performance liquid chromatography for the separation of open-cage fullerenes.