Enhancing the developmental competence of the early embryo using secretory leukocyte peptidase inhibitor

详细信息    查看全文

• 作者：Hyun Sook Kim^a ; Kwon-Soo Ha^b ; Hyeok Chan Kwon^c ; Seung Jae Lee^c ; Chung-Hoon Kim^d ; Yong-Pil Cheon^a ; ^{ypcheon@sungshin.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Secretory leukocyte peptidase inhibitor ; Developmental speed ; Implantation ; Competence
• 刊名：Differentiation
• 出版年：2016
• 出版时间：July-August 2016
• 年：2016
• 卷：92
• 期：1-2
• 页码：24-34
• 全文大小：1774 K

文摘

Secretory leukocyte peptidase inhibitor (SLPI) plays a role in proliferation and differentiation via the autocrine and paracrine systems. SLPI's expression is well-documented in the reproductive tract, but it remains unclear whether it is active during early embryonic development. In this study, the expression and role of m>Slpim> in the early embryo were evaluated. m>In vitrom> embryo cultures in chemically defined simple medium resulted in a reduction in developmental speed from the 8-cell stage, as well as implantation rate compared with m>in vivom> embryos. SLPI protein was localized to the membrane or submembrane cytoplasm in an embryonic stage-dependent manner. m>In vitrom> cultured embryos exhibited lower levels of m>Slpim> mRNA expression than m>in vivom> embryos. m>Slpim> knockdown by antisense oligonucleotides attenuated the developmental speed and implantation rate compared with m>Slpim> sense oligonucleotide-transfected embryos and m>in vitrom> controls. The critical period for the attenuation of developmental speed occurred after the 8-cell stage. SLPI treatment accelerated development, increased implantation rate, and ameliorated the suppressive effects of m>Slpim> knockdown. m>Slpim> knockdown did not induce changes in the total cell number or inner cell number in blastocysts. Meanwhile, SLPI upregulated the expression of the developmental factors matrix metalloproteinase-14, neutrophil elastase, and tissue inhibitor of metalloproteinase-1. Together, these results suggest that SLPI is an effective regulator of developmental speed and implantation competence in an autocrine and paracrine manner, respectively, and plays a role in controlling the expression of embryonic development factors, such as MMP family members.