- 作者：Xiayun Changa ; 1 ; Kai Zhangb ; 1 ; Rui Zhoua ; Fen Luoa ; Lingpeng Zhua ; Jin Gaoa ; He Hea ; Tingting Weia ; Tianhua Yana ; yantianhuabest@126.com" class="auth_mail" title="E-mail the corresponding author ; Chunhua Maa ;
- 关键词：NF-κB ; Nuclear factor-κB ; AMPK ; AMP-activated protein kinase ; IL-6 ; Interleukin (IL)-6 ; TNF-α ; tumor necrosis factor ; ELISA ; enzyme-linked immuno sorbent assay ; CK ; creatine kinase ; LDH ; lactate dehydrogenase ; TTC ; 2 ; 3 ; 5-triphenyltetrazoliumchloride
- 刊名：International Journal of Cardiology
- 出版年：2016
- 出版时间：15 July 2016
- 年：2016
- 卷：215
- 期：Complete
- 页码：532-544
- 全文大小：2842 K
Methods
Energy metabolism homeostasis and inflammation injury were respectively assessed in global ischemia of isolated hearts and coronary artery ligated rats.
Results
Excessive release of cardiac enzymes and pro-inflammatory cytokines was inhibited by salidroside in coronary artery occlusion-induced rats. ST segment was also restored with the treatment of salidroside. Triphenyltetrazolium chloride staining (TTC) staining and pathological analysis showed that salidroside could significantly alleviate myocardial injury in vivo. Accumulated data in ex vivo indicated that salidroside improved heart function recovery, which was reflected by enhanced myocardial contractility and coronary flow in isolated hearts. The contents of ATP and glycogen both in ex vivo and in vivo were restored by salidroside compared with those in the model group. Besides, the expressions of p-AMPK, PPAR-α and PGC-1α in rats and isolated hearts subjected to salidroside were significantly elevated, while the levels of p-NF-κBp65, p-IκBα, p-IKKα and p-IKKβ were dramatically reduced by salidroside.
Conclusions
The present study comprehensively elaborated the protective effects of salidroside on myocardial injury and demonstrated that AMPK/PGC-1α and AMPK/NF-κB signaling cascades were implicated in the myocardial ischemia–reperfusion injury (I/R) model.