Enhanced arbovirus surveillance with deep sequencing: Identification of novel rhabdoviruses and bunyaviruses in Australian mosquitoes
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- 作者：Lark L. Coffey ; Brady L. Page ; Alex ; er L. Greninger ; Belinda L. Herring ; Richard C. Russell ; Stephen L. Doggett ; John Haniotis ; Chunlin Wang ; Xutao Deng ; Eric L. Delwart
- 关键词：ABLV ; Australian bat lyssavirus ; AHSV ; African horse sickness virus ; AINOV ; Aino virus ; AKAV ; Akabane virus ; ALFV ; Alfuy virus ; ALMV ; Almpiwar virus ; BANV ; Banna virus ; BASV ; Bas-Congo virus ; BEAUV ; Beaumont virus ; BEFV ; bovine ephemeral fever virus ; BFV ; Barmah Forest virus ; BYSM ; barley yellow striate mosaic ; CCHFV ; Crimean-Congo hemorrhagic fever virus ; CHIKV ; chikungunya virus ; CHVV ; Charleville virus ; CPV ; Coastal Plains virus ; DAff sigma virus ; Drosophila affinis sigma virus ; DENV ; dengue
- 刊名：Virology
- 出版年：5 January, 2014
- 年：2014
- 卷：448
- 期：Complete
- 页码：146-158
- 全文大小：2158 K
文摘
Viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. A metagenomics approach was used to identify virus sequences in Australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. Sequence comparisons revealed strains of Liao Ning virus (Reovirus, Seadornavirus), previously detected only in China, livestock-infecting Stretch Lagoon virus (Reovirus, Orbivirus), two novel dimarhabdoviruses, named Beaumont and North Creek viruses, and two novel orthobunyaviruses, named Murrumbidgee and Salt Ash viruses. The novel virus proteomes diverged by 鈮?0% relative to their closest previously genetically characterized viral relatives. Deep sequencing also generated genomes of Warrego and Wallal viruses, orbiviruses linked to kangaroo blindness, whose genomes had not been fully characterized. This study highlights viral metagenomics in concert with traditional arbovirus surveillance to characterize known and new arboviruses in field-collected mosquitoes. Follow-up epidemiological studies are required to determine whether the novel viruses infect humans.