A squalene synthase protein degradation method for improved sesquiterpene production in Saccharomyces cerevisiae

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• 作者：Bingyin Peng^a ; Manuel R. Plan^a ; ^b ; Panagiotis Chrysanthopoulos^a ; ^b ; Mark P. Hodson^a ; ^b ; Lars K. Nielsen^a ; Claudia E. Vickers^a ; ^{c.vickers@uq.edu.au}
• 关键词：Sesquiterpene ; Trans-nerolidol ; Metabolic engineering ; Saccharomyces cerevisiae ; Endoplasmic reticulum-associated protein degradation (ERAD)
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：209-219
• 全文大小：928 K
• 卷排序：39

文摘

Squalene synthase was modulated by a protein degradation mechanism. Squalene synthase C-terminal peptide is an ER-targeting transmembrane signal peptide. A mechanism was engineered to degrade ER membrane-integrating protein. Enterococcus mvaS and mvaE were used to improve sesquiterpene production in yeast. Destabilizing squalene synthase redirects carbon flux to nerolidol production.