In vitro antimicrobial activities of metabolites from vaginal Lactobacillus strains against Clostridium perfringens isolated from a woman's vagina

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• 作者：Mansour Amin^a ; Parastoo Moradi Choghakabodi^b ; Mohammad Alhassan Hamidi^b ; Mahin Najafian^c ; Ahmad Farajzadeh Sheikh^b ; ^{Farajzadehah@gmail.com}
• 关键词：antimicrobial activity ; Clostridium perfringens ; Lactobacillus ; modified E test ; vagina
• 刊名：Journal of the Chinese Medical Association
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：80
• 期：1
• 页码：29-33
• 全文大小：586 K
• 卷排序：80

文摘

More than 50 different species of bacteria may live in a woman's vagina, with lactobacilli being the predominant microorganism found in healthy adult females. Lactobacilli are relevant as a barrier to infection and are important in the impairment of colonization by pathogens, owing to competitive adherence to adhesion sites in the vaginal epithelium and their capacity to produce antimicrobial compounds.MethodsThe aim of the present study was to demonstrate the inhibitory capability of Lactobacillus metabolites against Clostridium perfringens, an anaerobic Gram-positive bacterium. These bacteria were isolated from vaginal swabs by using culture-dependent approaches, and the bacteriostatic effect of Lactobacillus metabolites, extracted from different isolates, was assessed using a modified E test.ResultsAmong the 100 vaginal swabs, 59 (59%) samples showed the presence of Lactobacillus strains and only one sample contained C. perfringens. Lactobacillus metabolites demonstrated the significant potency of in vitro activity against C. perfringens, with minimal inhibitory concentration values ranging from 15.6 μg/mL to 31.2 μg/mL.ConclusionThis study suggests that women without vaginal Lactobacillus strains may be susceptible to nonindigenous and potentially harmful microorganisms.