Effective inhibition of bacterial respiration and growth by CuO microspheres composed of thin nanosheets

详细信息    查看全文

• 作者：Rizwan Wahab ; Shams Tabrez Khan ; Sourabh Dwivedi ; Maqusood Ahamed ; Javed Musarrat ; Abdulaziz A. Al-Khedhairy
• 关键词：X-ray diffraction pattern ; CuOMSs-Ths ; Microspheres ; Antibacterial activity ; Nanoparticles ; E. coli
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2013
• 出版时间：1 November, 2013
• 年：2013
• 卷：111
• 期：Complete
• 页码：211-217
• 全文大小：4130 K

文摘

This study describes the synthesis, characterization and biocidal potential of copper oxide micro-spheres composed of thin sheets (CuOMSs-Ths). Microscopic observations of synthesized CuOMSs-Ths revealed the clusters of thin sheets arranged in small flower like micro-spheres. Diameter of each micro-sphere was determined in the range of 2-3 ¦Ìm, whereas the size of each sheet was ¡«80 nm. These micro-flowers like nanostructures were synthesized using copper nitrate hexahydrate and sodium hydroxide via solution process. The CuOMSs-Ths exhibited a broad-spectrum anti-bacterial activity involving significant growth inhibition of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Micrococcus luteus. The IC₅₀ values of these engineered NPs against E. coli, P. aeruginosa, S. aureus and M. luteus were determined to be 195, 200, 131 and 184 ¦Ìg/ml, respectively. Also, the respiration of Gram+ ve organisms (M. luteus and S. aureus) was inhibited significantly (p value <0.005) at relatively lower concentrations of 12.5 and 50 ¦Ìg/ml, respectively, as compared to the Gram? ve bacteria (E. coli and P. aeruginosa), where the growth inhibition occurred at a much greater concentration of 100 ¦Ìg/ml. The results explicitly demonstrated anti-microbial activity of CuOMSs-Ths with a higher level of toxicity against the Gram+ ve vis-a-vis Gram? ve bacteria.