Phylogenetics of the millipede genus Brachycybe Wood, 1864 (Diplopoda: Platydesmida: Andrognathidae): Patterns of deep evolutionary history and recent speciation

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• 作者：Michael S. Brewer^a ; Chad L. Spruill^a ; Nandita S. Rao^a ; Jason E. Bond^b ; ^{jbond@auburn.edu}
• 关键词：Millipede phylogeny ; Myriapoda ; Speciation ; Species boundaries ; Ancestral range reconstruction ; GMYC
• 刊名：Molecular Phylogenetics and Evolution
• 出版年：2012
• 出版时间：July, 2012
• 年：2012
• 卷：64
• 期：1
• 页码：232-242
• 全文大小：1810 K

文摘

The genus Brachycybe Wood is a little known group of millipedes comprising eight nominal species distributed throughout North America, Japan, South Korea, Taiwan, and China. The group¡¯s species are relatively morphologically homogenous and have been described primarily on the basis of differences in somatic morphology largely ignoring the often-used characters in millipede taxonomy and systematics - male genitalia (the gonopods). The objectives of this study were to survey male gonopods with the aim of evaluating inter-specific variation, assess existing species boundaries and phylogeny using molecular characters, examine the historical biogeography of the genus, and estimate the timing of lineage divergence using a molecular clock. We surveyed two mitochondrial genes (cytochrome c oxidase I and cytochrome b) and one nuclear protein-coding gene (glutamyl and prolyl-tRNA synthetase) each comprising 952, 746, and 555 aligned base pairs respectively. Phylogenetic inference coupled with an analysis of species delimitation using a generalized mixed Yule coalescent model recovered eight species, two of which were considered to be cryptic. Molecular dating analyses coupled with ancestral range reconstructions indicate that the group is quite ancient (age > 50 million years) and its origins are likely traced back geographically to the mountains of California¡¯s Sierra Nevada and Coastal Ranges. The genus Brachycybe appears to have expanded its range at least twice out of present day California into eastern North America and at least once into Asia. This study highlights the need for integrative approaches to describe biodiversity and furthers the evidence for cryptic diversity even in groups where genitalia are generally thought to be rapidly evolving, diagnostic features.