Optimization of microchip-based electrophoresis for monoclonal antibody product quality analysis revealed needs for extra surfactants during denaturation
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- 作者：Hui Caia ; 1 ; Yuanli Songa ; 1 ; Jian Zhanga ; Ting Shia ; Ya Fub ; Rong Lia ; Nesredin Mussaa ; Nesredin.Mussa@BMS.com" class="auth_mail" title="E-mail the corresponding author ; Zheng Jian Lia
- 关键词：BME ; 2-mercaptoethanol ; CDR ; complementary determining regions ; CMC ; critical micelle concentration ; HC ; heavy chain ; IAM ; iodoacetamide ; LC ; light chain ; mAb ; monoclonal antibody ; NGHC ; non-glycosylated heavy chain ; PNGase F ; peptide N-glycosidase F ; TCEP ; tris 2-carboxyethyl phosphine HCl
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：20 February 2016
- 年：2016
- 卷：120
- 期：Complete
- 页码：46-56
- 全文大小：3010 K
文摘
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Optimize the micro-chip based electrophoresis condition of eight different mAbs.
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Elucidate denaturation profiles and SDS concentration required by different mAbs.
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Rationalize different response of mAbs to SDS using molecular modeling.
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