Fluorescent rare earth solutions as intrinsic wavelength standards for protein fluorescence spectroscopy

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• 作者：Heinz Anderle ; Alfred Weber ; ^{alfred.weber@shire.com}
• 关键词：Spectrofluorimeter ; Instrument validation ; Gadolinium ; Thulium ; Dysprosium
• 刊名：Analytical Biochemistry
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：518
• 期：Complete
• 页码：86-88
• 全文大小：196 K
• 卷排序：518

文摘

Trivalent Gd, Tm, and Dy solutions can be used as intrinsic excitation and emission standards to validate the UV and violet-blue wavelength accuracy of a spectrofluorimeter. Europium extends the range into the red. To attain sufficient sensitivity, these luminescent rare earth ions require deuterated reagents or carbonate complexation, which allow the use of ordinary water and thus preparation in virtually any laboratory. Such solutions are particularly valuable as system suitability standards (SST) for protein fluorescence spectroscopy to detect red shifts of the intrinsic fluorescence maximum in stability and storage studies.